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目的 构建人白介素 2 linker PE38融合基因 ,为进一步表达具有特异性杀伤作用的融合蛋白奠定基础。方法 用PCR方法扩增绿脓杆菌外毒素 (PEA)衍生物PE38基因及柔性连接肽linker片段 ,并与人白介素 2 (Interleukin2 ,IL2 )基因连接插入质粒pET2 8a中。结果 构建的表达载体经核苷酸测序分析无突变发生。结论 成功地构建了表达质粒pIL2 linker Pe38。
Objective To construct human interleukin 2 linker PE38 fusion gene and lay the foundation for further expression of a fusion protein with specific killing effect. Methods The PE38 gene and flexible linker peptide of Pseudomonas exotoxin (PEA) were amplified by polymerase chain reaction (PCR) and ligated with the Interleukin 2 (IL2) gene into plasmid pET2 8a. Results The constructed expression vector showed no mutation by nucleotide sequencing. Conclusion The expression plasmid pIL2 linker Pe38 was successfully constructed.