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目的 :观察胃癌癌前病变 (PL GC)组织中表皮生长因子受体 (EGFR)蛋白及 m RNA水平的表达及中药胃炎消的调控作用。方法 :胃镜病理证实为 PL GC病例共 4 0例 ,分为胃炎消治疗 (胃炎消 )组 2 5例 ,维酶素治疗(对照 )组 15例 ,治疗前、后胃粘膜固定部位活检标本 ,采用 L SAB免疫组织化学及原位分子杂交标记的方法作EGFR蛋白翻译和 m RNA转录水平的检测。结果 :胃炎消组症状总有效率为 84 .0 0 % ,病理疗效以中度异型增生为好 ,作用优于对照组 ;在 PL GC组织中 ,EGFR蛋白翻译和 m RNA转录水平均有过表达 ,且其表达随病变的进展而递增 ;胃炎消能使 EGFR蛋白及 m RNA的表达下调。结论 :胃炎消对 PL GC有良好的治疗作用 ;该治疗作用可能与本方下调 PL GC组织中与细胞增殖相关 EGFR蛋白及 m RNA的表达有关。
OBJECTIVE: To observe the expression of epidermal growth factor receptor (EGFR) protein and m RNA in gastric cancer precancerous lesions (PL GC) and the role of Chinese medicine gastritis. Methods: Gastroscopy was confirmed by PL GC in 40 cases. There were 25 cases of gastritis treated with gastritis and 15 cases treated with verapamil. The biopsy specimens of gastric mucosa were collected before and after treatment. EGFR protein translation and m RNA transcript levels were detected by L SAB immunohistochemistry and in situ hybridization. Results: The total effective rate of gastritis elimination group was 84.0%, and the pathological effect was moderate dysplasia, which was better than the control group. In PL GC tissues, both EGFR protein translation and m RNA transcript levels were over-expressed , And its expression increased with the progress of the lesion; Gastritis can reduce the expression of EGFR protein and m RNA. CONCLUSION: Gastritis can effectively treat PL GC. The therapeutic effect may be related to the down-regulation of the expression of EGFR protein and m RNA associated with cell proliferation in PL GC tissues.