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该文通过对忍冬叶初生及次生代谢过程进行研究,探讨不同发育阶段忍冬叶代谢规律。在每月下旬采收金银花上部5~8片完全展开叶,采用HPLC测定相关代谢指标。结果表明,忍冬叶含水量在3月份最高,达到78.59%,12月份最低,为60.83%;总糖量在冬季最高,1月份达到最高275.8 mg·g~(-1),其他时间明显降低;忍冬叶中的绿原酸、新绿原酸、木犀草苷和咖啡酸的含量变化基本一致,四者的含量均在3月达到最高,分别为42.79,2.01,7.13,0.16 mg·g~(-1)。忍冬叶的初生代谢产物和次生代谢产物含量在3—5月达到较高水平,与有效成分含量相关的因素主要有钾、镁、磷、门冬氨酸、苏氨酸、脯氨酸、缬氨酸、胱氨酸、异亮氨酸和苯丙氨酸。
In this paper, the primary and secondary metabolic processes of Lonicera japonica Thunb were studied to investigate the metabolic regulation of Lonicera japonica L. At different developmental stages. In the second half of each month, honeysuckle upper part of the upper 5 to 8 fully expanded leaves, using the determination of related metabolic indicators HPLC. The results showed that the water content of leaves of honeysuckle was the highest in March, reaching 78.59%, the lowest in December was 60.83%. The total sugar content was the highest in winter and reaching the highest in 275.8 mg · g -1 in January, The contents of chlorogenic acid, neogenic chlorogenic acid, luteolin and caffeic acid in honeysuckle leaves were basically the same, the content of four reached the highest in March, which were respectively 42.79, 2.01, 7.13 and 0.16 mg · g ~ (- 1). The content of primary metabolites and secondary metabolites in honeysuckle leaves reached a higher level from March to May. The main factors related to the content of active ingredients were potassium, magnesium, phosphorus, aspartic acid, threonine, proline, Valine, cystine, isoleucine and phenylalanine.