Objective To research the effect of nervous peptide on senile mice’ s immunological function at sub - low temperature environment. Methods Senile mice models were prepared with D galactose. Assayed the content of B - EP and L- EK、 proliferation response of splenic cell induced by ConA and LPS and the activities of IL - 1 and IL - 2 in senile mice as well. Results The content of β EP in plasma was obviously higher in control group and senile group of 10℃ or 5℃ than that in chamber temperature group (P ＜ 0.01), the content of L- EK was higher in control group 5℃ than that in control group 10 ℃ and control chamber temperature group ( P ＜ 0.01 or P ＜ 0.05); at chamber temperature, the content of β - EP was also higher in senile mice than that in control group and lower than that in control group at 5℃ (P ＜ 0.01). The proliferation response induced by ConA and LPS- two kinds of splenic cells, was obviously lower in group 10℃ and 5 ℃ than that in chamber temperature group ( P ＜ 0.01 ), the proliferation response induced by ConA or LPS in senile group 10℃ were higher than in control group (P＜ 0.01). Compared with chamber temperature group,the IL- 1 activity of senile mice of 5℃ group and control mice of 10℃ and 5 ℃ group increased distinctively ( P ＜ 0.01 ). Conclusion At 10℃ or 5 ℃temperature, the increasing of β - EP content and IL - 1 activity in senile mice had inhibited for the function of non - specific cell immunity and B cell.