１材料与方法１．１病原菌越冬存活与分生孢子萌发病原菌采自河北省地区玉米病株上,经分离、纯化后ＰＤＡ斜面保存;孢子萌发检测:将保存与斜面上的病原菌分别置于室内外不同场所和不同时间处理后,自培养基上洗下病原菌的分生孢子,滴于凹形载玻片上,２５℃或不同温度培养,２４ｈ 1 Materials and methods 1.1 Survival of pathogens overwintering and spore germination Pathogenic bacteria collected from Hebei province maize diseased strains, after separation, purified PDA slant preservation; spore germination test: the preservation and slant on the pathogen were placed indoors After treatment in different places and at different times, the pathogen conidia was washed from the culture medium, dropped on a concave glass slide and incubated at 25 ° C or at different temperatures for 24 h