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为探讨复方丹参丸对糖尿病大鼠肾脏病变的防治作用,将36只SD大鼠随机分成正常对照组、模型组与滴丸组。复方丹参滴丸(50mg/kgd-1)混入普通饲料给药;实验第90、180天分别测24h尿蛋白量,尿微量白蛋白及尿NAG并经尾静脉取血测血清尿素氮(BUN)、肌酐(Scr)。实验结束时颈动脉取血测血浆一氧化氮(NO)、丙二醛(MDA);全血谷胱甘肽过氧化物酶(GSH-PX)、组织型纤溶酶原激活物(t-PA)、纤溶酶原激活剂抑制物(PAI)等,并进行肾脏光镜及透射电镜观察。结果3个月时滴丸组尿微量白蛋白显著低于模型组(P<0.05)。6个月时滴丸组24h尿蛋白显著低于模型组(P<0.01),尿NAG、微量白蛋白,血尿素氮(BUN)、肌酐(Scr)等也有显著性差异(P<0.05)。6个月 时模型组大鼠出现明显高凝、高粘现象,肾脏微血管病变比较明显,复方丹参滴丸干预后高凝、高粘现象及微血管病变得到明显改善。结论:复方丹参滴丸可延缓或减轻糖尿病大鼠肾脏病变的发生发展。
To investigate the preventive and therapeutic effects of Fufang Danshen Pill on diabetic rats’ kidney disease, 36 SD rats were randomly divided into normal control group, model group and dropping pill group. Compound Danshen Dripping Pills (50mg/kgd-1) were mixed with common feeds; the amount of 24-hour urinary protein, urine microalbumin, and urinary NAG were measured on the 90th and 180th days of the experiment and blood was measured via the tail vein to measure serum urea nitrogen (BUN). Creatinine (Scr). At the end of the experiment, carotid blood was taken to measure plasma nitric oxide (NO) and malondialdehyde (MDA); whole blood glutathione peroxidase (GSH-PX) and tissue plasminogen activator (t- PA), plasminogen activator inhibitor (PAI), etc., and renal light and transmission electron microscopy observations. Results Urinary microalbumin was significantly lower in the dripping pill group than in the model group at 3 months (P<0.05). At 24 months, the 24h urinary protein in the dropping pill group was significantly lower than that in the model group (P<0.01), and the urinary NAG, microalbumin, blood urea nitrogen (BUN), and creatinine (Scr) were also significantly different (P<0.05). At 6 months, the rats in the model group showed marked hypercoagulability and hyperviscosity, and the renal microvascular lesions were more obvious. The hypercoagulability, hyperviscous phenomena and microvascular lesions were significantly improved after the intervention of Compound Danshen Dripping Pills. Conclusion: Compound Danshen Dripping Pills can delay or reduce the occurrence and development of diabetic nephropathy in rats.