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目的:探讨老龄大鼠局灶性脑缺血后CyclinD1表达与小胶质细胞活化、神经元凋亡之间的内在关系。方法:建立光化学诱导的老龄大鼠局灶性脑缺血模型,利用原位分子杂交、IsolectinB4免疫组织化学和原位末端标记等方法,检测缺血4、24 h和3、5 d组大鼠脑组织中的CyclinD1 mRNA阳性细胞、小胶质细胞和凋亡细胞的时空分布规律。结果:CyclinD1 mRNA阳性细胞、小胶质细胞和凋亡细胞皆分布于缺血灶周围,发布空间相互重叠。CyclinD1 mRNA在多种形态细胞中表达,以胶质细胞最为多见,其表达高峰与小胶质细胞活化高峰一致。在各时间点的病灶周围皆发现TUNEL阳性细胞,其高峰时点提前于CyclinD1 mRNA阳性细胞及小胶质细胞活化高峰。结论:CyclinD1可能参与了缺血后小胶质细胞的活化及神经元的凋亡过程。
Objective: To investigate the relationship between the expression of CyclinD1 and the activation of microglia and neuronal apoptosis after focal cerebral ischemia in aged rats. Methods: A rat model of focal cerebral ischemia induced by photochemistry was established. By means of in situ molecular hybridization, IsolectinB4 immunohistochemistry and in situ terminal labeling, the rats were sacrificed at 4, 24 and 35 d after ischemia The spatial and temporal distribution of CyclinD1 mRNA positive cells, microglial cells and apoptotic cells in brain tissue. Results: CyclinD1 mRNA positive cells, microglial cells and apoptotic cells were all distributed around the ischemic foci. The distribution space overlapped each other. CyclinD1 mRNA is expressed in a variety of morphological cells, the most common glial cells, the expression peak and microglia activation peak. TUNEL-positive cells were found around the lesion at each time point, the peak of CyclinD1 mRNA positive cells and microglial activation peak at the peak time point. Conclusion: CyclinD1 may be involved in the activation of microglia and neuronal apoptosis after ischemia.