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目的：研究一氧化氮合酶抑制剂Ｎ－硝基－Ｌ－精氨酸甲基酯对大鼠肠系膜淋巴管作用。方法：股静脉注入Ｎ－硝基－Ｌ－精氨酸甲基酯，活体动态观察其２小时内淋巴管运动的变化；用ＨＥ染色、酶组化染色研究其组织学改变。结果：发现静注Ｎ－硝基－Ｌ－精氨酸甲基酯可使淋巴管径变细，运动频率增加，Ｌ－精氨酸可暂时逆转该变化；镜下发现淋巴管内皮细胞及管壁上神经纤维都含有一氧化氮合酶阳性反应产物，注Ｎ－硝基－Ｌ－精氨酸甲基酯组小肠组织ＨＥ切片出现固有层中央乳糜管扩张。说明一氧化氮合酶在淋巴循环中可能具有重要调节作用。
AIM: To investigate the effect of nitric oxide synthase inhibitor N-nitro-L-arginine methyl ester on rat mesenteric lymphatic vessels. Methods: N-nitro-L-arginine methyl ester was injected into the femoral vein to observe the changes of the lymphatic vessels within 2 hours. The histological changes were observed by HE staining and enzyme histochemical staining. Results: It was found that intravenous injection of N-nitro-L-arginine methyl ester can make the diameter of lymphatic vessels thinner and increase the frequency of exercise. L-arginine can reverse this change temporarily. The lymphatic endothelial cells and tubes Nerve fibers on the wall contain nitric oxide synthase positive reaction products, note N-nitro-L-arginine methyl ester group of intestinal tissue HE section of central lamina propria central dilatation. That nitric oxide synthase may have an important regulatory role in the lymphatic circulation.