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目的探讨双蜡块检测胃癌中HER-2蛋白的表达,分析该方法的实用性。方法收集259例胃癌手术标本,每个病例用2个蜡块检测HER-2蛋白表达,统计学分析双蜡块和单蜡块免疫表型的差异。结果所有病例1号蜡块中HER-2呈1+、2+和3+分别为64例(24.7%)、100例(38.6%)、29例(11.2%)。2号蜡块中HER-2呈1+、2+、和3+分别为54例(20.8%)、106例(40.9%)、38例(14.7%)。联合1号和2号蜡块,HER-2呈1+、2+和3+分别为45例(17.4%)、122例(47.1%)、40例(15.4%)。1号和2号蜡块检测结果不一致为75例,占29.0%。结论在胃癌手术切除标本中,用双蜡块检测HER-2蛋白表达,能提高HER-2的阳性率,减少肿瘤异质性造成的假阴性。
OBJECTIVE: To investigate the expression of HER-2 protein in gastric cancer by double wax block and to analyze its practicability. Methods Twenty-five specimens of gastric cancer were collected. The expression of HER-2 protein was detected by two wax blocks in each case. The differences of immunophenotype between the two wax blocks and single wax block were statistically analyzed. Results In all the cases, the number of HER-2 in the No.1 wax block was 64 cases (24.7%), 100 cases (38.6%) and 29 cases (11.2%). There were 54 cases (20.8%), 106 cases (40.9%) and 38 cases (14.7%) of HER-2 in No. 2 wax block. There were 45 cases (17.4%), 122 cases (47.1%) and 40 cases (15.4%) of HER-2 in the combination of No.1 and No.2 wax blocks respectively. The inconsistency of test results of No.1 and No.2 wax blocks was found in 75 cases (29.0%). Conclusion The detection of HER-2 protein expression by double wax in surgical specimens of gastric cancer can increase the positive rate of HER-2 and reduce the false negative caused by tumor heterogeneity.