[Objective] Plant mutants have been widely used in gene function analysis. T-DNA insertion is a promising approach for mutant library construction. This study aimed to construct a multifunctional T-DNA insertion vector for generating mutant libraries of Salvia miltiorrhiza Bunge.[Methods] By using pBI121 as a starting vector,an intron sequence from S. miltiorrhiza was introduced. Four enhancers were directionally connected by digestion with Ban I restriction endonuclease. GUS gene promoter was removed and GUS gene was moved forward to construct the recombinant vector pBI-INTRON-GUS-4E.[Results] The constructed recombinant vector pBI-INTRON-GUS-4E harbors an intron sequence from S.miltiorrhiza,four enhancers,GUS gene and NPTII gene,which exhibits multiple functions such as activation tagging,gene(promoter) trapping and insertional mutagenesis.[Conclusions]The successfully constructed multifunctional T-DNA insertion vector provided the basis for generating mutant libraries of S.miltiorrhiza and other plant species. This study aimed to construct a multifunctional T-DNA insertion vector for generating mutant libraries of Salvia miltiorrhiza Bunge. [Methods ] By using pBI121 as a starting vector, an intron sequence from S. miltiorrhiza was introduced. Four enhancers were directionally connected by digestion with Ban I restriction endonuclease. GUS gene promoter was removed and GUS gene was moved forward to construct the recombinant vector pBI- INTRON-GUS-4E. [Results] The constructed recombinant vector pBI-INTRON-GUS-4E harbors an intron sequence from S. miltiorrhiza, four enhancers, GUS gene and NPTII gene, which exhibits multiple functions such as activation tagging, gene ) trapping and insertional mutagenesis. [Conclusions] The successfully constructed multifunctional T-DNA insertion vector provided the basis for generating mutant libraries of S. miltiorrh iza and other plant species.