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目的研究人类间充质干细胞(hMSC)成瘤过程中细胞运动能力及迁移能力的变化。方法依次应用人端粒酶逆转录酶(hTERT)、SV40 T抗原(SV40 TAg)及癌基因H-Ras V12转染hMSC成瘤,应用划痕愈合实验及Boyden小室实验分别检测成瘤过程中不同转化细胞的自由迁移和趋触性的改变。结果 hMSC成功转化为两种不同表型的恶性肿瘤细胞。细胞的自由迁移能力在全部成瘤过程中没有发生明显改变。转染RAS后,细胞的趋触性有明显增加。结论癌基因RAS可明显增加hMSC的迁移能力,永生化的hMSC在细胞运动能力方面相对安全。
Objective To study the changes of cell motility and migration ability of human mesenchymal stem cells (hMSC) during tumorigenesis. Methods HMSCs were transfected with human telomerase reverse transcriptase (hTERT), SV40 T antigen (SV40 TAg) and oncogene H-Ras V12 in turn. The wound healing test and Boyden chamber test were used to detect the tumorigenesis Transformed cells are free to migrate and change in touch. Results hMSC successfully transformed into two different phenotypes of malignant tumor cells. The ability of cells to freely migrate did not change significantly during the entire tumorigenesis. After transfected with RAS, the cell’s contact ability is obviously increased. Conclusion The oncogene RAS can significantly increase the migration ability of hMSCs, and the immortalized hMSCs are relatively safe in cell motility.