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目的：检测Ｂ７基因在肾细胞癌的表达及其与肾细胞癌分级、分期及预后的关系。方法：本文应用ＲＴＰＣＲ和免疫组织化学技术检测了２２例肾细胞癌组织Ｂ７的表达。结果：ＲＴＰＣＲ５例阳性，免疫组化４例阳性，Ｂ７ｍＲＮＡ表达与肾癌病理分级、分期无关系；病理切片ＨＥ染色显示，Ｂ７阳性肿瘤组织内淋巴细胞浸润明显增多。结论：表达Ｂ７的肿瘤组织局部抗肿瘤免疫反应增强，提示Ｂ７表达可能与肿瘤预后有关。大部分泌尿系肿瘤不表达Ｂ７，为转Ｂ７基因治疗泌尿系肿瘤提供了理论依据。
Objective: To detect the expression of B7 gene in renal cell carcinoma and its relationship with the grade, stage and prognosis of renal cell carcinoma. Methods: RT-PCR and immunohistochemistry were used to detect the expression of B7 in 22 cases of renal cell carcinoma. Results: RTPCR was positive in 5 cases and positive in 4 cases by immunohistochemistry. The expression of B7 mRNA was not related to the pathological grade and stage of renal cell carcinoma. HE staining showed that the infiltration of lymphocytes in B7 positive tumor increased obviously. Conclusion: Local anti-tumor immune response of B7-expressing tumor tissue is enhanced, suggesting that B7 expression may be related to tumor prognosis. Most urological tumors do not express B7, which provides a theoretical basis for the treatment of urinary tumors with B7 gene therapy.