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目的 :探讨P糖蛋白在骨肉瘤组织中的表达和其临床意义 ,以及与PCNA、P53 、nm -2 3蛋白表达的相关性。方法 :应用免疫组化方法 (S -P法 )测定P糖蛋白在 3 3例骨肉瘤组织中的表达。男 2 4例 ,女 9例 ,年龄平均 2 0 .6岁。ⅡA5例 ,ⅡB2 3例 ,ⅢB5例。同期采用相同方法测定PCNA、P53 、nm -2 3的表达。结果 :19例获得平均 1年 7个月随访 ,复发 3例 ,肺转移 11例 (其中 6例死亡 )。P糖蛋白阳性表达率为 45 .5 % ,与性别、年龄、部位及外科分期无关 ,与预后有关 ;与PCNA、P53 、nm -2 3蛋白表达无相关性。结论 :P糖蛋白的表达是骨肉瘤产生多药耐药的重要因素 ,并影响患者的预后 ,癌基因在骨肉瘤中的表达无相关性。
Objective: To investigate the expression of P-glycoprotein in osteosarcoma, its clinical significance, and the correlation with PCNA, P53 and nm-2 3 protein expression. Methods: The expression of P-glycoprotein in 33 osteosarcoma tissues was detected by immunohistochemical method (S-P method). There were 24 males and 9 females with an average age of 20.6 years. There were 5 cases of IIA, 3 cases of IIB2, and 5 cases of IIIB. The same method was used to determine the expression of PCNA, P53 and nm -2 3 in the same period. Results: 19 cases were followed up for an average of 1 year and 7 months. There were 3 cases of recurrence and 11 cases of lung metastases (including 6 deaths). The positive expression rate of P glycoprotein was 45.5%, which was not related to gender, age, location and surgical stage. It was related to prognosis. There was no correlation between PCNA, P53 and nm-22.3 protein expression. Conclusion : The expression of P glycoprotein is an important factor for the multidrug resistance of osteosarcoma and affects the prognosis of patients. There is no correlation between the expression of oncogene in osteosarcoma.