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目的研究我国T2DM人群AMPKα2基因多态性与冠心病(CAD)风险的相关性。方法以326例T2DM患者为研究对象,其中180例伴有CAD(CAD+组),146例不伴CAD(CAD组)。应用聚合酶链式反应—限制性内切酶片断长度多态性(PCR-RFLP)技术或基因测序方法 ,研究AMPKα2基因8个单倍型标记单核苷酸多态性(tag-SNPs)与CAD风险的关系。结果 (1)SNP rs11206887 GG基因型携带者较非携带者发生CAD的风险显著增加(OR=2.507,95%CI=1.244~5.053,P=0.010),校正年龄、性别、BMI、吸烟、糖尿病病程后仍存在统计学差异(OR’=2.469,95%CI’=1.182~5.157,P’=0.016)。(2)SNP rs2143749 GG基因型携带者较非携带者发生CAD的风险增高(OR=1.680,95%CI=1.029~2.741,P=0.038)。(3)SNP rs2746347 TT基因型携带者较非携带者发生CAD的风险有增高趋势(OR=2.875,95%CI=1.034~7.996,P=0.043,校正OR’=1.715,95%CI’=1.016~2.895,P’=0.044)。(4)SNP rs2143749和SNP rs11206887 GG/GG基因型组合携带者较非携带者发生CAD的风险增高(P=0.014)。结论我国T2DM患者AMPKα2 SNPs与CAD的发病风险可能相关。
Objective To investigate the association between AMPKα2 gene polymorphisms and risk of coronary heart disease (CAD) in T2DM population in China. Methods A total of 326 patients with T2DM were enrolled. Among them, 180 patients had CAD (CAD + group) and 146 patients had no CAD (CAD group). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique or gene sequencing method was used to study the association of 8 haplotype tag SNPs in AMPKα2 gene with CAD risk relationship. Results (1) SNP rs11206887GG genotype carriers had significantly higher risk of developing CAD than non-carriers (OR = 2.507, 95% CI = 1.244-5.053, P = 0.010). Age, sex, BMI, smoking, duration of diabetes There was still statistical difference (OR ’= 2.469, 95% CI’ = 1.182 ~ 5.157, P ’= 0.016). (2) SNP rs2143749 GG genotype carriers had a higher risk of developing CAD than non-carriers (OR = 1.680, 95% CI = 1.029-2.741, P = 0.038). (3) The risk of CAD in SNP rs2746347 TT genotype carriers was higher than that of non-carriers (OR = 2.875,95% CI = 1.034-7.996, P = 0.043, OR = 1.715, 95% CI = 1.016 ~ 2.895, P ’= 0.044). (4) The risk of CAD in SNP rs2143749 and SNP rs11206887 GG / GG genotype carriers was higher than that in non-carriers (P = 0.014). Conclusions AMPKα2 SNPs in T2DM patients in China may be associated with the risk of CAD.