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目的探讨肾炎四味片对肾组织纤维化的影响及作用机制。方法采用单侧输尿管结扎(unilateralureteral obstruction,UUO)肾间质纤维化模型,将雄性SD大鼠60只随机分为,①假手术组(A组);②肾炎四味片假手术组(B组);③模型组(C组),行左侧输尿管结扎术;④肾炎四味片治疗组(D组)。每组15只,各组分别于术后第3、7、14天处死5只大鼠,采用HE和Masson染色观察大鼠肾脏组织病理改变,应用免疫组织化学方法检测肾小管间质中转化生长因子-β1(transforming growth factor-β1,TGF β1)、核因子κB(nuclear factor-κB,NF-κB)的表达情况。结果 A组和B组各指标组内及组间比较差异无统计学意义(P>0.05)。病理变化,C组间质纤维化程度进行性加重,组内比较差异有统计学意义(P<0.0)5),D组间质胶原纤维面积明显低于相应时间点的C组,差异有统计学意义(P<0.05)。免疫组织化学,C组NF-KB、TGF-β1在肾间质表达明显增强,与A组相比差异有统计学意义(P<0.05),与间质胶原纤维面积呈正相关。D组各因子的表达下调,与C组相应时间点相比差异有统计学意义(P<0.05)。结论肾炎四味片可下调NF-κB、TGF-β1在肾间质中的表达,从而具有延缓肾间质纤维化的作用。
Objective To investigate the effect of Shenyin Siwei Tablet on renal fibrosis and its mechanism. Methods Sixty male Sprague Dawley rats were randomly divided into sham operation group (A group), unilateral ureteral obstruction (UUO) group, sham operation group (B group) ); Model group (C group), the left ureteral ligation; ④ Shenyimitai tablets treatment group (D group). The rats in each group were sacrificed on the 3rd, 7th and 14th day after operation. Five rats were sacrificed on the 3rd, 7th and 14th day after operation. The pathological changes of the kidney were observed by HE and Masson staining. The expressions of transforming growth in the tubulointerstitium were detected by immunohistochemistry Β1 (TGFβ1) and nuclear factor-κB (NF-κB) were detected by Western blot. Results There was no significant difference between groups A and B (P> 0.05). (P <0.05). The area of interstitial collagen fibers in group D was significantly lower than that in group C at the corresponding time points, the difference was statistically significant Significance (P <0.05). The expression of NF-KB and TGF-β1 in renal interstitium in group C was significantly higher than that in group A (P <0.05), and positively correlated with the area of interstitial collagen fibers. Compared with the corresponding time points in group C, the differences of the expression of various factors in group D were statistically significant (P <0.05). Conclusion Shen Yan Siwei Tablet can downregulate the expression of NF-κB and TGF-β1 in the renal interstitium, which has the effect of retarding renal interstitial fibrosis.