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目的探索p16基因转染对胰腺癌细胞生长辐射敏感性的作用。方法将外源野生型p16基因连接到pCDNA3·1+载体构建pCDNA3·1+-p16重组质粒,利用LipofectamineTM介导转染胰腺癌JF305细胞,筛选阳性克隆。照射后,RT-PCR检测p16基因表达,Western blot检测蛋白表达;用MTT法测定细胞生长曲线和肿瘤细胞杀伤率。结果转染p16基因的JF305细胞有外源p16基因的整合及表达,生长速度明显减少,对辐射的敏感性增强。结论导入外源野生型p16基因可抑制胰腺癌细胞恶性增殖,提高胰腺癌细胞对辐射的敏感性。
Objective To explore the effect of p16 gene transfection on the radiation sensitivity of pancreatic cancer cells. Methods The exogenous wild-type p16 gene was ligated into pCDNA3.1 + vector to construct pCDNA3.1 + -p16 recombinant plasmid. LipofectamineTM was used to transfect pancreatic cancer JF305 cells and the positive clones were screened. After irradiation, the expression of p16 gene was detected by RT-PCR and the protein expression was detected by Western blot. The cell growth curve and tumor cell killing rate were measured by MTT assay. Results Transfection of p16 gene in JF305 cells with exogenous p16 gene integration and expression, the growth rate was significantly reduced, the sensitivity of radiation increased. Conclusion The introduction of exogenous wild-type p16 gene can inhibit the malignant proliferation of pancreatic cancer cells and enhance the sensitivity of pancreatic cancer cells to radiation.