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目的:探讨多氯联苯(PCB)对大鼠睾丸组织表型及表达c-fos、c-Myc和β-catenin的影响。方法:Wistar大鼠45只随机分为4组,对照组饲喂正常饲料,低、中、高剂量组分别饲喂不同PCB含量的颗粒饲料(分别含PCB0.1、1、10mg/kg),90d后建立慢性PCB毒性动物模型。利用组织病理学技术分析PCB对大鼠睾丸组织表型的影响;采用免疫组化方法检测睾丸组织c-fos、c-Myc和β-catenin的表达。结果:PCB染毒组睾丸组织病理学表现为睾丸水肿,间质表型受损,生精小管形态发生改变,间质细胞、初级精母细胞、次级精母细胞消失,精子数量少见。1mg/kg和10mg/kg组c-fos和c-Myc的表达明显强于对照组和0.1mg/kg组(P<0.01);0.1mg/kg组β-catenin蛋白表达下调,与其它各组相比差异显著(P<0.01);1mg/kg组β-catenin的表达高于对照组和10mg/kg组(P<0.05)。结论:PCB能引起大鼠睾丸组织表型的改变,c-fos、c-Myc和β-catenin的异常表达与PCB致睾丸损伤有密切关系。
Objective: To investigate the effects of PCBs on the phenotype of testis and the expression of c-fos, c-Myc and β-catenin in rats. Methods: Forty-five Wistar rats were randomly divided into 4 groups. The control group was fed with normal diet. The low, middle and high dose groups were fed with different PCB contents of pellet feed (PCB0.1,1,10 mg / kg, respectively) After 90 days, a chronic animal model of PCB toxicity was established. The histopathology was used to analyze the effect of PCB on the testicular tissue phenotype in rats. Immunohistochemistry was used to detect the expression of c-fos, c-Myc and β-catenin in testis. Results: The histopathology of testis in test group showed testicular edema, impaired interstitial phenotype, morphological changes of seminiferous tubules, interstitial cells, primary spermatocytes and secondary spermatocytes disappeared and the number of sperm was rare. The expressions of c-fos and c-Myc in 1mg / kg and 10mg / kg groups were significantly higher than those in control and 0.1mg / kg groups (P <0.01) (P <0.01). The expression of β-catenin in 1 mg / kg group was higher than that in control group and 10 mg / kg group (P <0.05). CONCLUSION: PCB can induce the change of testicular tissue phenotype in rats. The abnormal expressions of c-fos, c-Myc and β-catenin are closely related to the testicular damage induced by PCB.