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为了解决百日咳杆菌感染早期诊断不能及时准确的难题 ,研制了测定百日咳特异性抗体的酶联免疫吸附试验 (ELISA)诊断试剂盒。经采用 9份疑似百日咳病人的血清、30 9份未接种过百白破混合制剂 (DPT)的婴幼儿的阴性血清、2 93份接种过DPT的婴幼儿的阳性血清验证 ,证明ELISA诊断试剂盒测定百日咳特异性抗体的敏感性和准确性都高于常规的凝集试验 ,能达到临床诊断快速、灵敏的效果。
In order to solve the problem that the early diagnosis of Bordetella pertussis infection can not be timely and accurately, an ELISA kit for detecting pertussis-specific antibody was developed. Positive serum samples from 9 sera from suspected patients with pertussis, 309 infants with no DPT (DPT) and 2 93 infants with DPT vaccination proved that the ELISA diagnostic kit Determination of pertussis specific antibody sensitivity and accuracy are higher than the conventional agglutination test, to achieve rapid and sensitive clinical diagnosis.