A frequency mixing and phase locking system is designed, in which electromagnetic shielding, microwave coupling, and intermediate frequency (IF) measurement arrangements are included. In lieu of liquid nitrogen, a pulse tube cryocooler is used to cool the whole system. With Josephson grain boundary junction as the mixing element, the 96th harmonic frequency mixing at 3 mm waveband is obtained, and phase-locked voltage- controlled oscillator (VCO) is realized.