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目的和方法：采用体外细胞培养，进行氚标胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入和原位杂交。结果：发现γ－干扰素（ＩＦＮ－γ）能抑制体外培养新西兰兔血管平滑肌细胞（ＶＳＭＣ）的ＤＮＡ合成和血小板衍化生长因子Ａ链（ＰＤＧＦ－Ａ）的基因表达；整体实验中，用４ＦＦｏｇａｒｔｙ导管气囊拉伤新西兰兔的腹主动脉内皮后，随即连续肌注ＩＦＮ－γ（１６５万Ｕ·ｋｇ１·ｄ１）３天，结果发现肌注ＩＦＮ－γ能明显抑制受损部位ＶＳＭＣ的３Ｈ－ＴｄＲ的标记率及ＰＤＧＦ－Ａ的表达。结论：ＩＦＮ－γ对血管成形术引起的血管挛缩有一定的舒张作用。
PURPOSE AND METHODS: T cell thymidine (3H-TdR) incorporation and in situ hybridization were performed in vitro. Results: IFN-γ was found to inhibit DNA synthesis and PDGF-A gene expression in cultured New Zealand rabbit vascular smooth muscle cells (VSMCs) in vitro. In the whole experiment, 4FF logarty catheters After balloon injury of the abdominal aorta in New Zealand rabbits, IFN-γ (165,000 U · kg-1 · d-1) was intramuscularly injected for 3 days. The results showed that intramuscular IFN-γ significantly inhibited the damaged site 3H-TdR labeling rate and PDGF-A expression in VSMCs. CONCLUSION: IFN-γ can relax vasoconstriction induced by angioplasty.