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目的探讨环氧合酶(COX)-2及肝细胞凋亡在非酒精性脂肪性肝炎(NASH)发生中的作用。方法雄性SD大鼠30只,随机分为模型组、乙醇灌胃对照组和正常对照组,每组各10只。模型组给予高脂饮食(普通饲料基础上加10%猪油、2%胆固醇和5%蛋黄粉)。乙醇灌胃对照组在普通饲料中加乙醇灌胃喂养。正常对照组给予普通饲料。动物喂养12周后处死并取肝脏组织,用免疫组化法及逆转录(RT)-PCR法检测COX-2表达,TUNEL法观察肝细胞凋亡。结果模型组(0.97±0.32)和乙醇对照组(0.89±0.19)大鼠COX-2表达水平均明显高于正常对照组(0),差异有统计学意义(P<0.01)。模型组(3.26±0.90)和乙醇对照组(3.33±0.78)的肝细胞凋亡指数明显高于正常对照组(0.80±0.65),差异亦有统计学意义(P<0.01)。模型组与乙醇对照组之间COX-2表达和肝细胞凋亡指数差异均无统计学意义(P>0.05)。结论COX-2在NASH发生中发挥着重要作用,肝细胞凋亡是NASH发生的重要机制。
Objective To investigate the role of cyclooxygenase (COX) -2 and hepatocellular apoptosis in the development of non-alcoholic steatohepatitis (NASH). Methods Thirty male SD rats were randomly divided into model group, ethanol administration group and normal control group, with 10 rats in each group. The model group was given a high-fat diet (10% lard, 2% cholesterol and 5% egg yolk powder on the basis of normal diet). Ethanol was given to the control group in the normal feed plus ethanol fed. Normal control group given ordinary feed. After 12 weeks of feeding, the animals were sacrificed and liver tissues were taken out. The expression of COX-2 was detected by immunohistochemistry and RT-PCR, and the apoptosis of hepatocytes was observed by TUNEL. Results The expression of COX-2 in model group (0.97 ± 0.32) and ethanol control group (0.89 ± 0.19) was significantly higher than that in normal control group (0), the difference was statistically significant (P <0.01). The apoptotic index of model group (3.26 ± 0.90) and ethanol control group (3.33 ± 0.78) were significantly higher than that of the normal control group (0.80 ± 0.65), the difference was also statistically significant (P <0.01). There was no significant difference in COX-2 expression and hepatocyte apoptosis index between the model group and the ethanol control group (P> 0.05). Conclusion COX-2 plays an important role in the pathogenesis of NASH. Hepatocyte apoptosis is an important mechanism of NASH.