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目的:优选江南卷柏提取物的石油醚脱脂工艺,为该植物的资源开发与利用提供参考。方法:以穗花杉双黄酮提取量和浸膏得率为评价指标,通过正交试验考察石油醚用量、提取时间和提取次数对脱脂工艺的影响。采用HPLC测定穗花杉双黄酮含量,流动相乙腈(A)-水(B)梯度洗脱(0~5 min,25%A;5~12 min,25%~35%A;12~17 min,35%~45%A;17~25 min,45%~50%A;25~40 min,50%~55%A;40~45 min,55%~70%A;45~50 min,70%~100%A;50~55 min,100%A;55~60 min,100%~25%A),检测波长330 nm。结果:最佳脱脂工艺为加6倍量石油醚回流提取2次,每次1 h;穗花杉双黄酮提取量72.77 mg,浸膏得率11.04%。结论:该脱脂工艺稳定可行,适用于江南卷柏提取物的纯化。
OBJECTIVE: To optimize the petroleum ether degreasing process of Selaginella polygamum extract, which can provide reference for the exploitation and utilization of the plant resources. Methods: The extraction of biflavonoids from Fraxinus mandshurica and the yield of extract were used as evaluation indexes. The effects of petroleum ether dosage, extraction time and extraction times on the degreasing process were investigated by orthogonal test. The content of biflavone in Fraxinus mandshurica was determined by HPLC. The mobile phase was eluted with a gradient of acetonitrile-water (B) (0-5 min, 25% A, 5-12 min, 25-35% A, 12-17 min, 35 to 45% A; 17 to 25 minutes, 45 to 50% A; 25 to 40 minutes, 50 to 55% A; 40 to 45 minutes, 55 to 70% ~ 100% A; 50-55 min, 100% A; 55-60 min, 100% ~ 25% A). The detection wavelength is 330 nm. Results: The best degreasing process was 6 times refluxing extraction of petroleum ether 2 times, 1 h each; biflavum flavone extraction 72.77 mg, extraction rate 11.04%. Conclusion: The degreasing process is stable and feasible, and it is suitable for the purification of the extract of Selaginella dahurica.