论文部分内容阅读
为研究转基因作物的土壤生态安全性,于根际箱中种植2组大豆(A组:转基因品系OE-8、OE-7、RNAi-3和受体南农88-1;B组:转基因品系Gaga1 17-4、Gaga1 21-8、Gaga1 57和受体N2899),采集大豆花期根际土壤,分析硫元素含量和芳基硫酸酯酶活性,并采用Biolog微平板法,研究相比于受体,转基因品系对土壤微生物群落功能多样性的影响。结果表明:OE-8和RNAi-3与其受体相比,根际土壤硫元素含量显著降低;2组组内对比结果显示,根际土样芳基硫酸酯酶活性差异均不显著。Biolog GN/GP/FF/ECO系统及多样性指数分析显示:A组中RNAi-3根际土壤微生物群落活性显著低于受体,微生物群落的丰富度、多样性和均度显著降低,OE-7根际土壤微生物群落的丰富度和均度显著降低;B组中Gaga1 57和Gaga1 17-4根际土壤微生物群落的丰富度、多样性和均度显著下降。
In order to study the soil ecological safety of genetically modified crops, two groups of soybean were planted in the rhizobox (Group A: transgenic lines OE-8, OE-7, RNAi-3 and acceptor Nannon 88-1; Group B: transgenic lines Gaga1 17-4, Gaga1 21-8, Gaga1 57 and receptor N2899) were collected to collect rhizosphere soils at the flowering stage of soybean, the content of sulfur and the activity of aryl sulfatase were analyzed. Biolog microplate method Effects of Transgenic Lines on Functional Diversity of Soil Microbial Communities. The results showed that the contents of sulfur in rhizosphere soil of OE-8 and RNAi-3 were significantly decreased compared with their counterparts. Comparing the results of two groups, there was no significant difference in the aryl sulfatase activity of rhizosphere soil. Biolog GN / GP / FF / ECO system and diversity index analysis showed that the microbial community activity of RNAi-3 rhizosphere soil was significantly lower than that of the recipient in group A, the richness, diversity and homogeneity of microbial community were significantly decreased, while the OE- The soil microbial community in rhizosphere soil had significantly lower abundance and homogeneity of soil microbial biomass in the rhizosphere soil than in the rhizosphere soils. The abundance, diversity and homogeneity of microbial communities in the rhizosphere of Gaga1 57 and Gaga1 17-4 decreased significantly in the B group.