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Objective:To explore the influence of extract of Ginkgo biloba(EGB) on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma(ACC) of lacrimal gland.Methods:ACC-2 cell in human with ACC of lacrimal gland was in vitro cultured. MTT method was used for cell proliferation detection.Annexin V/PI double-staining flow cytometer was used to detect cell apoptosis and cell cycle.Survivin gene expression was analyzed by RT-PCR and Western blotting.Results:EGB had inhibitory effect on the proliferation of ACC-2 cell with significant dose-effect relationship,and there was statistical difference when compared with the control group(P<0.01).The inhibitory concentration 50%(IC_(50)) is 88 mg/L. The flow cytometer test indicated that CGB can gradually increase ACC-2 cell in G_0-G_1 stage and decrease it in G_2-M and S stage.With the increase of dose,the apoptosis rate of ACC-2 cell was obviously increased(P<0.05 or P<0.01).EGB had certain inhibitor)’ effect on Survivin gene expression of ACC-2 cell,and Survivin gene expression was decreased with the increasing of the EGB concentration(P<0.01).Conclusions:EGB can effectively inhibit Survivin gene expression of ACC-2 cell in human with ACC of lacrimal gland,induce the apoptosis of ACC-2 cell and inhibit tumor cell proliferation.
Objective: To explore the influence of extract of Ginkgo biloba (EGB) on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma (ACC) of lacrimal gland. Methods: ACC-2 cell in human with ACC of The assay was used for cell proliferation detection. Annexnex V / PI double-staining flow cytometer was used to detect cell apoptosis and cell cycle. Survivin gene expression was analyzed by RT-PCR and Western blotting. Results: EGB had inhibitory effect on the proliferation of ACC-2 cells with significant dose-effect relationship, and there was statistical difference when compared with the control group (P <0.01). The inhibitory concentration 50% (IC 50) was 88 mg / L. The flow cytometer test indicated that CGB can gradually increase ACC-2 cell in G_0-G_1 stage and decrease it in G_2-M and S stage .With the increase of dose, the apoptosis rate of ACC-2 cell was been increased (P <0.05 or P <0.01). EGB had certain inhibitor) ’effect on Survivin gene expression of ACC-2 cell, and Survivin gene expression was decreased with increasing of EGB concentration (P <0.01) .Conclusions: EGB can inhibit survivin gene expression of ACC-2 cell in human with ACC of lacrimal gland , induce the apoptosis of ACC-2 cell and inhibit tumor cell proliferation.