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小麦(Triticum aestivumL.)品系‘兰考90(6)’在2AL染色体臂上携带1个隐性抗白粉病基因,暂命名为PmLK906。以‘中国春’/‘兰考90(6)21-12’杂交F3代纯合株系构建的抗、感cDNA池为探针进行小麦基因芯片杂交,结果表明与粗山羊草(Aegilops tauschii)csAtPR5类似的基因在抗病池中的表达量比感病池中约高24.2倍。随后,从‘兰考90(6)21-12’中成功克隆了一个2 125 bp的全长csAtPR5-类似基因cDNA序列,暂命名为TaAetPR5(GenBank登录号:EU082094)。TaAet-PR5与csAtPR5的cDNA序列有93%的相同。TaAetPR5编码1个由657个氨基酸组成的多肽,与csAtPR5有88%的相同。蛋白推导分析表明TaAetPR5的分子量为74.5 kDa,pI 6.82,可能是一个可溶性的球蛋白,存在于细胞核中的可能性是95%。接种小麦白粉病菌(Blumeria graminisf.sp.tritici)后12至72 h,‘兰考90(6)21-12’幼苗叶片中的TaAetPR5基因转录水平不断提高。感白粉病的‘中国春’中没有检测到TaAetPR5基因。
Wheat (Triticum aestivum L.) line Lankao 90 (6) ’carries a recessive powdery mildew resistance gene on the 2AL chromosome arm and is tentatively named PmLK906. The anti-sense and sense cDNA pools constructed from F3 generation homozygous lines of ’Chinese Spring’ / ’Lankao 90 (6) 21-12’ were used as probes for wheat gene chip hybridization. The results showed that Aegilops tauschii csAtPR5 Similar genes expressed 24.2 times more potent than in susceptible pools. Subsequently, a full-length cDNA sequence of 2 125 bp of csAtPR5-like gene was successfully cloned from Lankao 90 (6) 21-12 ’and named as TaAetPR5 (GenBank accession number: EU082094). The cDNA sequence of TaAet-PR5 and csAtPR5 is 93% identical. TaAetPR5 encodes a polypeptide of 657 amino acids, 88% identical to csAtPR5. Protein deduction analysis showed that TaAetPR5 had a molecular weight of 74.5 kDa, pI 6.82, probably a soluble globulin with a 95% chance of being present in the nucleus. The level of TaAetPR5 gene transcription in ’Lankao 90 (6) 21-12’ seedling leaves was continuously increased from 12 to 72 h after inoculation with Blumeria graminis sp. Tritici. TaAetPR5 gene was not detected in ’Chinese Spring’ of powdery mildew.