论文部分内容阅读
目的研究丁苯酞对大鼠体内细胞色素P450酶2C19(cytochrome P450 enzyme 2C19,CYP2C19)活性的影响。方法 Wistar大鼠随机分为4组,每组8只。对照组灌胃给予植物油;实验组分为低、中、高3个剂量组,分别灌胃给予丁苯酞40、80、160mg/kg(以植物油溶解)。对照组和实验组均连续灌胃5d,于第6天尾静脉注射探针药物奥美拉唑(4mg/kg)后由眼底静脉丛取血,血浆样品经二氯甲烷提取,以卡马西平为内标,采用高效液相色谱法测定奥美拉唑血药浓度。以DAS 2.1.1药物动力学程序拟合药动学参数。结果对照组与实验组比较,奥美拉唑药动学参数AUC_((0-1))、AUC_((0-∞))、t_(1/2)、CL_Z、V_Z差异无统计学意义(P>0.05)。结论丁苯酞对大鼠体内CYP2C19酶活性无影响。
Objective To investigate the effect of butylphthalide on cytochrome P450 enzyme 2C19 (CYP2C19) activity in rats. Methods Wistar rats were randomly divided into 4 groups with 8 rats in each group. The control group was given gavage with vegetable oil. The experimental group consisted of low, medium and high dose groups, which were given 40, 80 and 160 mg / kg Butylphthalide respectively. The control group and the experimental group were fed continuously for 5 days. On the 6th day after the intravenous injection of the probe drug omeprazole (4 mg / kg), the blood was collected from the fundus of the fundus. The plasma samples were extracted with methylene chloride and carbamazepine As internal standard, the determination of omeprazole plasma concentration using high performance liquid chromatography. Pharmacokinetic parameters were fitted using the DAS 2.1.1 pharmacokinetic program. Results There was no significant difference in pharmacokinetic parameters AUC_ (0-1), AUC_ ((0-∞)), t_ (1/2), CL_Z and V_Z between the control group and the experimental group P> 0.05). Conclusion Butylphthalide has no effect on CYP2C19 activity in rats.