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建立了高效液相色谱-波长切换法同时测定清宫长春胶囊中的尿囊素(1)、去氢土莫酸(2)、土莫酸(3)、茯苓酸(4)、梓醇(5)、吉奥诺苷B_1(6)、马替诺皂苷(7)、人参皂苷Rg_1(8)和人参皂苷Rb_1(9)。采用Agilent TC-C_(18)色谱柱;以乙腈∶0.2%磷酸为流动相,线性梯度洗脱;切换波长检测:224 nm(0→19 min,1)、210 nm(19→31 min,2~5)、330 nm(31→37 min,6、7)、203 nm(37→55 min,8、9)。1~9分别在8.0~160.0、2.0~40.0、2.5~50.0、2.0~40.0、5.0~100.0、2.5~50.0、2.0~40.0、15.0~300.0和8.5~170.0μg/ml范围内线性关系良好;平均回收率分别为98.82%、100.03%、97.92%、98.09%、97.64%、98.14%、96.91%、97.88%和98.39%,RSD<2%。
A method for the simultaneous determination of allantoin (1), dehydroartemoic acid (2), trimellitic acid (3), pachyrin acid (4), catalpol ), Ginonoside B_1 (6), manitinoside (7), ginsenoside Rg_1 (8) and ginsenoside Rb_1 (9). The mobile phase was eluted with a linear gradient of acetonitrile: 0.2% phosphoric acid using a mobile phase of Agilent TC-C 18 with a wavelength of 224 nm (0 → 19 min, 1), 210 nm (19 → 31 min, 2 ~ 5), 330 nm (31 → 37 min, 6,7), 203 nm (37 → 55 min, 8,9). 1 to 9 showed a good linearity in the range of 8.0 to 160.0, 2.0 to 40.0, 2.5 to 50.0, 2.0 to 40.0, 5.0 to 100.0, 2.5 to 50.0, 2.0 to 40.0, 15.0 to 300.0 and 8.5 to 170.0 μg / The recoveries were 98.82%, 100.03%, 97.92%, 98.09%, 97.64%, 98.14%, 96.91%, 97.88% and 98.39%, respectively. The RSDs were less than 2%.