论文部分内容阅读
本文对中国首例输入性D8基因型麻疹病毒基因特征进行分析。用ELISA法检测血清麻疹病毒IgM抗体;用Vero/Slam细胞对采集的咽拭子标本进行病毒分离,分离到的麻疹毒株用RT-PCR方法扩增其核蛋白基因3′端的部分序列,并对扩增产物进行核苷酸序列测定和分析,以3′端456个核苷酸为目的片段进行基因亲缘性关系分析。结果表明,上海市2012年共报告1 105疑似麻疹病例,其中实验室确诊590例,临床符合病例2例,排除513例,报告发病率为2.52/10万;共采集到984份疑似麻疹病例咽拭子标本,分离到247株麻疹病毒,病毒分离阳性率为25.3%;除Shanghai12-239为D8基因型外,其他均为H1a基因亚型。Shanghai12-239与世界卫生组织(World Health Organization,WHO)参考株(Manchester.UNK30.94(D8)AF280803)核苷酸序列同源性为97.8%,氨基酸序列同源性为98.6%。与WHO其他基因型参考株核苷酸序列同源性为89.6%~94.5%,氨基酸序列同源性为88.7%~95.3%。
This article analyzes the first imported genetic characteristics of D8 genotype measles virus in China. Serum measles virus IgM antibody was detected by ELISA method. Vero / Slam cells were used for virus isolation of the collected throat swab specimens. The isolated measles virus strain was amplified by RT-PCR from the 3 ’end of the nucleoprotein gene The amplified products were sequenced and analyzed. The 456 nucleotides at the 3 ’end were used to analyze the genetic relationship. The results showed that in 2012, 105 cases of suspected measles were reported in Shanghai, of which 590 cases were laboratory confirmed, 2 cases were clinically confirmed, 513 cases were excluded, and the reported incidence was 2.52 / 100 000. A total of 984 suspected measles cases were collected Swab specimens, 247 strains of measles virus were isolated. The positive rate of virus isolation was 25.3%. H112 subtype was all except D12 genotype of Shanghai12-239. Shanghai12-239 and the World Health Organization (WHO) reference strain (Manchester.UNK30.94 (D8) AF280803) showed 97.8% nucleotide sequence homology and 98.6% amino acid sequence identity. The nucleotide sequences of the reference strains of other WHO genotypes were 89.6% -94.5% with amino acid homologies of 88.7% -95.3%.