论文部分内容阅读
目的对沙枣总酚的辐射防护作用进行研究。方法采用不同剂量X射线照射人皮肤成纤维细胞(Human Skin Fibroblasts,HSF),构建辐射损伤模型,以MTT法检测不同剂量沙枣总酚对HSF增殖的影响。结果 X射线对HSF的增殖抑制作用与辐射剂量呈正相关。与2、4、8 Gy辐射组比较,16 Gy辐射组增殖抑制作用有显著差异(P<0.01)。采用16 Gy一次性X射线照射构建辐射损伤模型,模型组与不同剂量沙枣总酚组在2,24,48 h(除100μg/ml-48 h组以外)时段均有显著统计学差异(P<0.01)。照射后2 h,辐射防护作用呈现剂量依赖性,但各剂量给药组的辐射防护作用均随辐射后时间延长而减弱。结论 X射线辐射对HSF具有明显损伤作用,而沙枣总酚对其具有一定的保护作用。
Objective To study the radioprotective effect of total phenolics of jujube. Methods Human skin fibroblasts (HSF) were irradiated with different doses of X-rays to establish a radiation injury model. The effects of different doses of total phenols on the proliferation of HSF were detected by MTT assay. Results X-ray inhibition of HSF proliferation was positively correlated with radiation dose. Compared with 2,4,8 Gy radiation group, the proliferation inhibition effect of 16 Gy radiation group was significantly different (P <0.01). Radiation injury model was established by 16 Gy single-time X-ray irradiation. There was a significant statistical difference between the model group and the total phenolics group at 2, 24, 48 h (except 100 μg / ml-48 h group) (P <0.01). Radiation protection was dose-dependent at 2 h after irradiation, but the radioprotective effect of each dose group was weakened with the prolongation of irradiation time. Conclusion X-ray irradiation has a significant damage on HSF, while total phenolics of S. jujuba play a protective role.