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目的:探讨苍术炒焦前后对湿阻中焦模型大鼠水通道蛋白2(AQP2)和AQP3含量的影响,为苍术炒焦机制研究提供参考。方法:将动物分成空白组,湿阻中焦模型组,生苍术高、中、低剂量组(3 459.2,1 729.6,864.8 mg·kg~(-1))及焦苍术高、中、低剂量组(剂量同生品)和香砂胃苓丸阳性组(1 729.6 mg·kg~(-1)),连续灌胃给药7 d。利用紫外分光光度法和酶联免疫法分别检测给药前后大鼠尿液中AQP2含量和结肠黏膜中AQP3含量。结果:与空白组比较,模型组尿液中AQP2含量增加,结肠黏膜中AQP3含量降低,两者间的差异均具有极显著性意义。与模型组比较,各给药组大鼠尿液中AQP2含量均降低,且生品组AQP2含量较焦品组低;结肠黏膜中AQP3含量均增加,且焦品组含量高于生品组。结论:苍术的生品与焦品均有祛湿止泻作用,生苍术祛湿作用强于焦品,焦苍术止泻作用强于生品。
Objective: To investigate the effect of herb on the expression of AQP2 and AQP3 in rats with rheumatoid arthritis induced by scorching, and provide reference for the research on mechanism of scorching anxiety in herb. Methods: The animals were divided into blank group, wet-resistance middle focal model group, high, medium and low dose group (3 459.2, 1 729.6, 864.8 mg · kg -1) and high, medium and low dose Group (the same dose of raw material) and Xiangshaweiling capsules (1 729.6 mg · kg -1) were administered orally for 7 days. The contents of AQP2 in urine and the content of AQP3 in colonic mucosa were detected by UV spectrophotometry and enzyme-linked immunosorbent assay respectively. Results: Compared with the blank group, the content of AQP2 in the urine of the model group increased and the content of AQP3 in the colonic mucosa decreased. The difference between the two groups had extremely significant significance. Compared with model group, the content of AQP2 in urine of rats in each administration group decreased, and the content of AQP2 in raw product group was lower than that of coke product group. The content of AQP3 in colonic mucosa increased, and the content of coke product group was higher than that of raw product group. Conclusion: Both the raw and the coke products of Atractylodes macrophylla have the function of removing dampness and stopping diarrhea, the damp-removing effect of raw Cang Shu is stronger than that of coke products, and the anti-diarrhea effect of Jiao Astrake is stronger than that of raw products.