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目的探讨大蒜素(DT)诱导MDA-MB-231细胞凋亡的作用及其机制。方法用人乳腺癌细胞株MDAMB-231细胞随机分为正常对照组、DT组(于培养基中加入终质量浓度为20μg·mL-1 DT作用细胞48h)紫杉醇(PTX)组(PTX 1μg·mL-1)和DT+PTX组。MTT法检测细胞存活率,流式细胞术检测细胞凋亡率,RT-PCR和Western blot检测puma、bax及bcl-2基因的表达。结果 DT+PTX组MDA-MB-231细胞增殖抑制率明显高于DT组、PTX组[(57.8±2.85)%比(25.45±1.58)%、(29.86±2.33)%,P<0.01]。DT+PTX组MDA-MB-231细胞凋亡率明显高于DT组、PTX组[(53.5±3.25)%比(24.7±1.45)%、(27.8±2.26)%,P<0.01]。DT+PTX组细胞中PUMA、Bax蛋白上调及Bcl-2蛋白表达下调的程度大于DT组、PTX组(P<0.05)。结论 DT、PTX合用可显著提高对MDA-MB-231细胞增殖抑制作用,诱导凋亡,两药合用具有协同抗肿瘤作用。
Objective To investigate the effect and mechanism of garlicin (DT)-induced apoptosis in MDA-MB-231 cells. Methods Human breast cancer cell line MDAMB-231 cells were randomly divided into normal control group and DT group (20 μg·mL-1 DT-treated cells were added to the medium for 48 h) Taxol (PTX) group (PTX 1 μg·mL- 1) and DT+PTX group. The viability of the cells was detected by MTT assay, the apoptosis rate was detected by flow cytometry, and the expressions of puma, bax and bcl-2 genes were detected by RT-PCR and Western blot. Results The proliferation inhibition rate of MDA-MB-231 cells in DT+PTX group was significantly higher than that in DT and PTX groups [(57.8±2.85)% vs (25.45±1.58)%, (29.86±2.33)%, P<0.01]. The apoptosis rate of MDA+MB-231 cells in DT+PTX group was significantly higher than that in DT and PTX groups [(53.5±3.25)% vs (24.7±1.45)%, (27.8±2.26)%, P<0.01]. The degree of down-regulation of PUMA, Bax protein and down-regulation of Bcl-2 protein in DT+PTX group cells were greater than those in DT group and PTX group (P<0.05). Conclusion The combination of DT and PTX can significantly increase the inhibition of MDA-MB-231 cell proliferation and induce apoptosis. The combination of DT and PTX has a synergistic anti-tumor effect.