对丝状噬菌体的结构、基因组及生命周期的深入认识,是丝状噬菌体表面呈现技术建立和发展的基础。可将外源基因片段插入噬菌体的基因Ⅲ(g3)或基因Ⅷ(g8)的先导序列的紧下游,使外源基因表达的多肽以融合蛋白的形式呈现在噬菌体表面外壳蛋白gp3或gp8的N端,这样的呈现常能使表达的多肽保持生物活性,据此可用活的噬菌体直接方便、高效率地筛选目的基因或检测该基因产物的活性。这技术已被用于抗体基因文库、cDNA文库、随机多肽基因文库和突变基因文库的构建和筛选等方面。 The in-depth understanding of the structure, genome and life cycle of filamentous phage is the basis for the establishment and development of filamentous phage surface technology. The exogenous gene fragment can be inserted into the downstream of the leader of the gene of phage Ⅲ (g3) or gene Ⅷ (g8) to make the exogenous gene expressed polypeptide as the fusion protein in the phage surface coat protein gp3 or gp8 End, such a presentation can often maintain the biological activity of the expressed polypeptide, whereby the live phage can be used conveniently and efficiently to screen the target gene or to detect the activity of the gene product. This technique has been used in the construction of antibody gene libraries, cDNA libraries, random peptide gene libraries, and mutant gene libraries.