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利用两因素多水平析因试验设计，按Ｃｕ（０，０．０５，０．１，０．２，０．４ｍｇ／ｋｇ）和ＶＥ（０，１５０ｍｇ／ｋｇ）将６０只大鼠随机分成１０组，按组合条件饲养，７天后腹腔注射ＣＣｌ４，从血清酶学和组织酶学方面，探讨微量元素Ｃｕ和ＶＥ对ＣＣｌ４所致肝损伤的保护作用。结果表明：对ＳＯＤ、ＧＰＴ、ＧＯＴ３项指标单独Ｃｕ组和单独ＶＥ组与ＣＣｌ４组比较均有显著性差异（Ｐ＜０．０１），说明Ｃｕ和ＶＥ都对ＣＣｌ４所致肝损伤有保护作用。但对Ｃｕ来说，保护效果并不随剂量的增加而增强。同时，Ｃｕ和ＶＥ具有联合保护作用（Ｐ＜０．０１），当ＶＥ用量为１５０ｍｇ／ｋｇ时，Ｃｕ的用量为０．１ｍｇ／ｋｇ，取得最优保护效果
Sixty rats were randomly divided into 10 groups (Cu (0,0.05,0.1,0.2,0.4mg / kg) and VE (0,150mg / kg)) using the two factor and multi-level factorial design. The rats were fed with CCl4 intraperitoneally 7 days later. The protective effects of trace elements Cu and VE on liver injury induced by CCl4 were studied in terms of serum enzymology and histology. The results showed that there was a significant difference (P <0.01) between SOD, GPT and GOT3 indexes in Cu group and VE alone group compared with CCl4 group, indicating that both Cu and VE have a protective effect on CCl4-induced liver injury. However, for Cu, the protective effect did not increase with dose. At the same time, Cu and VE had a combined protective effect (P <0.01). When the dosage of VE was 150 mg / kg, the amount of Cu was 0.1 mg / kg, which achieved the best protection effect