[Objective]To optimize the microwave-assisted extraction technology of total flavonoids from FRUCTUS LYCII.[Method]Using rutin as reference substance,the factors that influenced microwave-assisted extraction of total flavonoids were optimized by means of single-factor test and orthogonal design test.[Result]The optimal extraction process of total flavonoids was as follows:70% aqueous ethanol solution,solid-liquid ratio 1:30(g /ml),microwave radiation time 8 min,microwave power 400 W,radiation temperature 120 ℃.In this condition,the yield of total flavonoids was 18.3 mg /g.[Conclusion]Compared with the traditional technology,microwave-assisted extraction is more efficient and easy-operating in extracting total flavonoids from FRUCTUS LYCII. [Objective] To optimize the microwave-assisted extraction technology of total flavonoids from FRUCTUS LYCII. [Method] Using rutin as reference substance, the factors that influenced microwave-assisted extraction of total flavonoids were optimized by means of single-factor test and orthogonal design The optimal extraction process of total flavonoids was as follows: 70% aqueous ethanol solution, solid-liquid ratio 1:30 (g / ml), microwave radiation time 8 min, microwave power 400 W, radiation temperature 120 ° C .In this condition, the yield of total flavonoids was 18.3 mg / g. [Conclusion] Compared with the traditional technology, microwave-assisted extraction is more efficient and easy-operating in total flavonoids from FRUCTUS LYCII.