CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTOR WITH GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR G

来源 :Chinese Journal of Cancer Research | 被引量 : 0次 | 上传用户:loseunit
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客观:构造真核细胞的表示向量那个快速的人的 granulocyte 巨噬细胞为做的刺激殖民地的因素(hGM-CSF ) 基因在哺乳动物的房间高度快速。方法:完全从导致的人的胎儿的肺(HFL ) 提取 RNA 房间线。HGM-CSF cDNA 被反向的抄写聚合酶链反应(RT-PCR ) 获得,然后方向性地 subcloned 进印地语和 pcDNA3.1 plasmid 上的 EcoRI 地点形成表示向量 pcDNA3.1-GM-CSF 的 recombinant。结果:PCR 扩大被识别,顺序被分析,结果
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