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目的:通过探讨Twist和基质金属蛋白酶2(MMP-2)在胃癌组织中的表达水平,以研究两者与胃癌临床病理因素的相互关系,从而为临床上胃癌的转移复发预警及预后评估提供更准确的研究靶点。方法:首先收集37例胃癌患者石蜡组织切片及其对应的临床病例资料,然后通过免疫组化的方法检测Twist和MMP-2在37例胃癌病理组织切片中的表达情况;通过统计分析进一步了解Twist和MMP-2与临床胃癌相关病理因素之间的关系,同时对两者之间表达水平的关联性进行分析。结果:Twist蛋白在肿瘤细胞的胞质中呈现高表达,同时也存在于细胞核中,其阳性表达率为56.8%;胃癌的淋巴结转移、浆膜浸润等主要与Twist基因的表达有关(P<0.05);MMP-2蛋白在肿瘤细胞的胞质中呈现高表达,同时也存在于细胞核中,其阳性表达率为54.1%;胃癌的复发及远处转移与MMP-2的表达有关(P<0.05);Twist和MMP-2在胃癌组织中的表达呈正相关(r=0.257,P<0.05)。结论:胃癌组织中Twist和MMP-2表达均存在异常,且两者的表达水平的变化与临床上胃癌的转移、复发等过程密切相关;同时在一定程度上胃癌组织中的Twist的表达水平可能会调控MMP-2的表达。
Objective: To investigate the relationship between Twist and matrix metalloproteinase 2 (MMP-2) in gastric cancer and to explore their correlation with the clinicopathological factors of gastric cancer, so as to provide more information for early warning and prognosis of metastasis and recurrence of gastric cancer Accurate research target. Methods: The paraffin sections of 37 gastric cancer patients and corresponding clinical cases were collected. Immunohistochemical staining was used to detect the expression of Twist and MMP-2 in 37 gastric cancer tissue sections. The statistical analysis was used to further understand Twist And the relationship between MMP-2 and pathological factors related to clinical gastric cancer, and to analyze the correlation between the expression level of the two. RESULTS: Twist protein was highly expressed in the cytoplasm of the tumor cells and also present in the nucleus. The positive rate of Twist protein was 56.8%. The expression of Twist gene was mainly associated with lymph node metastasis and serosal invasion in gastric cancer (P <0.05) ). MMP-2 protein was highly expressed in the cytoplasm of tumor cells and was also present in the nucleus. The positive rate of MMP-2 protein was 54.1%. The recurrence and distant metastasis of gastric cancer were related to the expression of MMP-2 (P <0.05 ). The expression of Twist and MMP-2 in gastric cancer tissues was positively correlated (r = 0.257, P <0.05). Conclusions: The expressions of Twist and MMP-2 in gastric cancer are abnormal. The changes of the expression of Twist and MMP-2 in gastric cancer are closely related to the metastasis and recurrence of gastric cancer. The expression level of Twist in gastric cancer may be a certain extent Will regulate the expression of MMP-2.