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目的研究抗炎性细胞因子转化生长因子-β(TGF-β)、白细胞介素(IL-10)在三氯乙烯(TCE)致敏豚鼠血清和皮肤中的水平,探讨TCE接触过敏性皮炎的发病机制。方法选用雌性豚鼠74只,随机分成空白对照组、溶剂对照组、TCE处理组,用豚鼠最大值实验方法建立动物致敏模型,并在终末激发后把致敏豚鼠和未致敏豚鼠分别分为24 h、72h、1周、2周组并在相应时间点采集血样。采用酶联免疫吸附法检测各组血清中TGF-β、IL-10水平,免疫组织化学法检测皮肤组织中TGF-β、IL-10的表达。结果 TCE处理组致敏率为68.8%。血清TGF-β水平检测,致敏72 h和1周组比致敏24 h组明显升高(P<0.05),致敏72 h、1周组高于未致敏同时点组(P<0.05);血清IL-10水平检测,致敏72 h组比致敏24 h组明显升高(P<0.05),致敏2周组显著下降(P<0.05),致敏72 h组高于未致敏72 h组(P<0.05)。皮肤组织中TGF-β、IL-10的表达,致敏72 h组高于致敏24 h组、致敏1周组显著高于致敏72 h组(P<0.05),致敏2周组降至最低,致敏72 h、1周组高于未致敏同时点组(P<0.05)。结论 TCE对豚鼠皮肤具有强致敏作用,TGF-β、IL-10在TCE接触过敏性皮炎发生过程中可能具有重要意义。
Objective To study the levels of anti-inflammatory cytokines such as transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) in serum and skin of guinea pig sensitized with Trichlorethylene (TCE) Pathogenesis. Methods Seventy-four female guinea pigs were randomly divided into blank control group, solvent control group and TCE treatment group. The animal model of sensitization was established by guinea pig maximal experimental method, and sensitized guinea pigs and non-sensitized guinea pigs 24 h, 72 h, 1 week, 2 weeks and blood samples were collected at the corresponding time points. Serum levels of TGF-β and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA), and the expression of TGF-β and IL-10 in skin tissues were detected by immunohistochemistry. Results The sensitization rate of TCE treatment group was 68.8%. The level of serum TGF-βin the sensitized group was higher than that of the sensitized group for 24 h and 72 h (P <0.05) (P <0.05). The levels of IL-10 in sera at 72 h after sensitization were significantly higher than those at 24 h (P <0.05) Sensitized 72 h group (P <0.05). The expression of TGF-β and IL-10 in skin tissue was higher in sensitized 72 h group than in sensitized 24 h group, and was significantly higher in sensitized 1-week group than in sensitized 72-h group (P <0.05) To the lowest, sensitized 72 h, 1 week group was higher than the same time point group was not sensitized (P <0.05). Conclusion TCE has a strong sensitizing effect on guinea pig skin. TGF-β and IL-10 may play an important role in the process of TCE exposure to atopic dermatitis.