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目的探讨白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNFα)对大鼠肝星状细胞c-jun基因表达的影响。方法在培养的肝星状细胞系中加入IL-1β1μg/L、TNFα30μg/L,于8h、24h、48h、72h4个时间点收集细胞,提取总RNA;用逆转录定量PCR方法测定c-jun的基因表达水平。结果IL-1β组肝星状细胞c-jun基因表达水平在8h、24h、48h3个时间点均明显高于对照组;8h最高,为对照组的3倍。TNFα组肝星状细胞c-jun的基因表达水平在8h、24h、48h、72h均明显高于对照组;8h最高,为对照组的4倍。结论IL-1β、TNFα可增强肝星状细胞c-jun基因的表达。
Objective To investigate the effects of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNFα) on c-jun gene expression in rat hepatic stellate cells. Methods The cultured HSC cells were treated with IL-1β1μg / L and TNFα30μg / L, and the cells were harvested at 8h, 24h, 48h and 72h, and the total RNA was extracted. The c-jun Gene expression level. Results The expression of c-jun gene in hepatic stellate cells of IL-1β group was significantly higher than that of the control group at 8h, 24h and 48h, and reached the highest at 8h, which was three times of that of the control group. The gene expression levels of c-jun in hepatic stellate cells of TNFα group were significantly higher than that of the control group at 8h, 24h, 48h and 72h, the highest was 8h, which was 4 times of the control group. Conclusion IL-1β, TNFα can enhance the expression of c-jun gene in hepatic stellate cells.