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目的:探讨放射性n 125I粒子对肝癌裸鼠皮下移植瘤微血管形成的影响及其机制。n 方法:构建人肝癌Huh7细胞裸鼠皮下移植瘤模型，将12只裸鼠用随机数表法分为观察组和对照组，每组6只。观察组移植瘤植入一枚活度为2.96×10n 7Bq粒子，对照组植入一枚活度为0的空粒子。每4天测量1次移植瘤体积，记录肿瘤生长曲线。28 d后取下移植瘤，用免疫组织化学染色法检测CD31评估移植瘤微血管密度(MVD)。用免疫组织化学染色法检测血管内皮生长因子-A(VEGF-A)、缺氧诱导因子1α(HIF-1α)蛋白表达，并做半定量分析。用实时荧光定量聚合酶链反应(RT-PCR)检测VEGF-A、HIF-1α mRNA的表达。n 结果:观察组移植瘤的生长速度较对照组缓慢，粒子植入后12 d两组肿瘤体积差异有统计学意义(n t＝3.167，n P<0.05)，随着观察时间延长，两组肿瘤体积差距加大，在28 d时两组肿瘤体积分别为(963.61±89.56)mmn 3和(602.10±75.98)mmn 3(n t＝7.122，n P<0.05)。两组移植瘤组织中CD31均有表达，观察组的MVD较对照组少，差异有统计学意义(n t＝6.360，n P<0.05)。观察组VEGF-A、HIF-1α mRNA及蛋白的表达水平均低于对照组，差异均有统计学意义(n t＝10.480、6.414、10.890、12.250，n P<0.05)。n 结论:放射性n 125I粒子通过降低肿瘤微血管密度抑制肝癌移植瘤的生长，其机制可能与VEGF-A、HIF-1α的mRNA及蛋白表达减少有关。n “,”Objective:To investigate the effect of radioactive n 125I seed on angiogenesis of subcutaneously transplanted hepatocellular carcinoma in nude mice and underlying mechanism.n Methods:The subcutaneous transplanted tumor model of human hepatocellular carcinoma Huh7 cells was established in nude mice. Twelve nude mice were randomly divided into observation group and control group with 6 mice in each group. The n 125I seed with activity of 2.96×10n 7Bq was implanted into the transplanted tumor of observation group and another with 0 Bq as control group, respectively. The volume of the transplanted tumor was measured every 4 d and the growth curve of the tumor was recorded. The microvessel density (MVD) of the transplanted tumor was evaluated by immunohistochemical detection of CD31. VEGF-A and HIF-1α protein and mRNA were detected by immunohistochemistry and RT-PCR, respectively.n Results:The growth rate of tumor in the observation group was slower than that in the control group, and the difference of tumor volume between two groups at 12 d after n 125I seed implantation was significantly different(n t=3.167, n P<0.05). At 28 d after transplantation, the tumor volumes of control and observation group approached to (963.61 ± 89.56) mmn 3and (602.10±75.98) mmn 3, respectively. The MVD of the observation group was significantly lower than that of the control group (n t=6.361, n P<0.05). The relative expression of VEGF-A and HIF-1α mRNA in the observation group was significantly lower than that in the control group (n t=10.480, 6.414, n P<0.05). Protein expression levels of VEGF-A and HIF-1α in the observation group were lower than those in the control group (n t=10.890, 12.250, n P<0.05).n Conclusions:Radioactive n 125I seed can inhibit the growth of HCC xenografts by reducing tumor microvessels, which may be related to the decrease of VEGF-A and HIF-1α expression.n