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目的:探讨利用γ鄄干扰素(IFN鄄γ)基因治疗对大肠癌及肝转移的预防和治疗作用,并初步探讨其作用机制。方法:利用BALB/C小鼠成瘤的小鼠结肠癌CT26细胞株制备小鼠结肠癌腹腔转移瘤模型,用携带鼠IFN鄄γ基因的重组缺陷型腺病毒AdIFN鄄γ进行治疗,同时利用携带LacZ基因的腺病毒AdLacZ和PBS作空白对照,检测经基因治疗后小鼠体内IFN鄄γ基因的表达情况、脾脏的细胞毒性T淋巴细胞(CTL)活性变化、肿瘤局部的淋巴细胞浸润情况、肝转移的发生及荷瘤小鼠的生存期。结果:经IFN鄄γ基因治疗后,与对照组相比,治疗组小鼠血清中IFN鄄γ表达量明显增加(P<0.01),脾脏的CTL活性明显增强(P<0.01),肿瘤生长受到抑制,肝转移的发生率明显下降,荷瘤小鼠的存活期明显延长。结论:利用IFN鄄γ基因治疗大肠癌具有明显的疗效,并对大肠癌肝转移具有一定的预防和治疗作用。
Objective: To investigate the preventive and therapeutic effects of IFN-γ gene therapy on colorectal cancer and liver metastasis and to explore its mechanism. Methods: The model of peritoneal metastasis of mouse colon cancer was established by using CT26 cell line of mouse colon carcinoma of BALB / C mice. The model of peritoneal metastases of mouse colon cancer was treated with recombinant adenovirus AdIFN γ carrying murine IFN γ gene. LacZ gene adenovirus AdLacZ and PBS as a blank control to detect the gene IFN γ gene expression in mice after gene therapy, cytotoxic T lymphocytes (CTL) activity changes in the spleen, tumor local lymphocyte infiltration, the liver The occurrence of metastasis and the survival of tumor-bearing mice. Results: Compared with the control group, IFN-γ expression in the treated group was significantly increased (P <0.01) and CTL activity in the spleen was significantly increased (P <0.01) Inhibition, the incidence of liver metastases decreased significantly, tumor-bearing mice significantly prolonged survival. Conclusion: The treatment of colorectal cancer with IFN-γ gene has obvious curative effect, and it has some preventive and therapeutic effects on liver metastasis of colorectal cancer.