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目的:建立先天性膈疝大鼠胎肺体外培养模型。方法:孕鼠Nitrofen灌胃,图像分析方法统计先天性膈疝大鼠胎肺体外培养模型的肺胚芽的发育参数(面积和周长),统计数值以(?)±s表示,并对结果进行t检验。结果:对照组与肺胚芽发育明显比Nitrofen组成熟,培养96h后,可见胚芽发育并增殖;Nitrofen组肺胚芽发育差,培养72h后肺胚芽逐渐出现发育停止和坏死的情况;96h后,两组肺发育参数肺面积[(2.535±0.423)mm~2 vs(1.053±0.033)mm~2]及周长[(6.544±0.710)mm vs(4.862±0.458)mm]相比均有统计学意义(P<0.05)。结论:Nitrofen在胚胎发育早期导致肺发育不良,适于先天性膈疝大鼠胎肺体外培养模型建立。
Objective: To establish an in vitro model of fetal lung of congenital diaphragmatic hernia in rats. Methods: Nitrofen gavage was performed in pregnant rats, and the developmental parameters (area and perimeter) of lung germ cells in fetal lung of rat congenital diaphragmatic hernia were calculated by image analysis. The statistical values were expressed as (±) s, and the results were t test. Results: The control group and the lung germ developed significantly more than the Nitrofen group. After 96 hours of culture, the embryo developed and proliferated. The lung germ of the Nitrofen group developed poorly. After 72 hours of culture, the development and necrosis of the lung germ gradually appeared. After 96 hours, The lung development parameters of lung area [(2.535 ± 0.423) mm ~ 2 vs (1.053 ± 0.033) mm ~ 2] and circumference [(6.544 ± 0.710) mm vs (4.862 ± 0.458) mm] P <0.05). Conclusion: Nitrofen causes lung dysplasia in the early stage of embryonic development and is suitable for the establishment of fetal lung in vitro model of congenital diaphragmatic hernia.