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近来关于用同位素减毒幼虫作血吸虫疫苗的研究进行很多,但最佳的放射量及接种数众说不一,本实验就此问题进一步探讨。WFFS和NMRI株(简称W株和N株)曼氏血吸虫均来源于波多黎哥株。N株尾坳用于感染非纯系瑞士小鼠,以此获得毛蚴,毛蚴再感染钉螺,将释出的尾蚴及获得的童虫(按Colley氏法)分别分为两组,每组用20和56krad γ射线照射(强度为4.4krad/分钟),然后将照射后尾蚴及童虫经皮肤分别感染CBA/HT6T6和C57BL/10小鼠。感染后4～5周
Recent studies on the use of isotopically attenuated larvae for schistosoma vaccination have been conducted in a large number, but the best dosages and vaccination numbers vary, and this issue is further explored in this lab. WFFS and NMRI strains (referred to as W strain and N strain) Schistosoma mansoni are derived from Puerto Rico strains. N strain of colostrum was used to infect non-pure Swiss mice to obtain miracidia and miracidia, which were then inoculated into the snails. The released cercariae and the obtained worms (Colley’s method) were divided into two groups with 20 And 56krad γ-ray irradiation (intensity of 4.4krad / min). Cercatus and Schistosoma japonicum were infected with CBA / HT6T6 and C57BL / 10 mice via the skin respectively. 4 to 5 weeks after infection