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目的探讨不同剂量六味地黄丸对自发性2型糖尿病(T2DM)模型小鼠肝脏、肾脏和胰腺组织学变化的影响。方法将6~8周龄自发性T2DM模型小鼠(KK-Ay小鼠)随机分为无药对照组、低剂量组和高剂量组,C57BL/6J小鼠作为遗传对照组。各组小鼠分别给予蒸馏水或六味地黄丸灌胃15 w。给药15 w后,HE染色观察小鼠肝脏、肾脏和胰腺组织学变化。结果给药组小鼠肝脏、肾脏和胰腺组织病变明显减轻。低剂量组对小鼠肝脏的保护效果优于高剂量组;高剂量组对肾脏病变的治疗效果好于低剂量组;低剂量组对胰岛内胰岛细胞具有明显的保护作用。结论六味地黄丸对自发性T2DM所致的实验小鼠肝脏、肾脏和胰腺组织病理性改变具有明显的改善作用,且与给药剂量相关。
Objective To investigate the effects of different doses of Liuweidihuang Pill on histological changes of liver, kidney and pancreas in mice with spontaneous type 2 diabetes (T2DM). Methods Six to eight-week-old spontaneous T2DM model mice (KK-Ay mice) were randomly divided into control group, low dose group and high dose group, C57BL / 6J mice as genetic control group. The mice in each group were given distilled water or Liuweidihuangwan orally for 15 w. After 15 w of administration, histological changes of liver, kidney and pancreas of mice were observed by HE staining. Results The liver, kidney and pancreas lesions in the treated group were significantly reduced. The protective effect of low-dose group on the liver of mice was better than that of high-dose group. The high-dose group was more effective than low-dose group on the treatment of renal lesions. Low-dose group had obvious protective effect on pancreatic islet cells. Conclusion Liuweidihuang Pill has a significant effect on the pathological changes of liver, kidney and pancreas in spontaneous T2DM mice, and it is related to the dose of Liuweidihuangwan.