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以乙烯受体基因LeETR1和LeETR2的反义转基因株系ale1和ale2及其杂交株系dale为材料,研究乙烯生物合成抑制剂AVG和CoCl2对这3个反义转基因表达株系器官衰老脱落特性的影响及转基因株系乙烯敏感性的变化。结果表明,经过AVG和CoCl2处理后,50%叶柄外植体的脱落时间延长4.6～6.2d,转基因株系与对照株系差异显著(p<0.05);50%花外植体的衰老时间延长2.4～3.9d,仅ale1和dale与对照株系差异显著(p<0.05)。研究结果提示番茄乙烯受体LeETR1和LeETR2在调节叶柄和花衰老脱落过程中所起作用不同。叶柄脱落由两个基因共同参与,花的衰老与LeETR2的关系不大;番茄植株在叶柄脱落过程中的乙烯敏感性与在花衰老过程中的乙烯敏感性不同,从而提示乙烯敏感性对不同的器官和过程有不同的意义。
The antisense transgene lines ale1 and ale2 of ethylene receptor genes LeETR1 and LeETR2 and their hybrid lines dale were used to study the effects of ethylene biosynthesis inhibitors AVG and CoCl2 on the decay characteristics of organ aging in three antisense transgenic lines Affect Variations of Ethylene Sensitivity in Transgenic Lines. The results showed that 50% petiole explants shed 4.6 ~ 6.2 days after treatment with AVG and CoCl2, the transgenic lines were significantly different from the control (p <0.05); 50% 2.4 ~ 3.9d, only ale1 and dale significant difference with the control line (p <0.05). The results suggest that the tomato ethylene receptors LeETR1 and LeETR2 play different roles in the regulation of petioles and flower senescence. Petiole shedding was mediated by two genes, and senescence of flowers had little relationship with LeETR2. The ethylene sensitivity of tomato plants in the process of petiole shedding was different from that of ethylene in the flower senescence process, which suggested that the sensitivity of ethylene to different Organ and process have different meanings.