论文部分内容阅读
目的 研究反义ClC 3寡核苷酸对thapsigargin诱导的细胞凋亡的影响。方法 蛋白免疫印迹法检测ClC 3蛋白的表达 ;MTT法检测反义ClC 3寡核苷酸对TG诱导的细胞生长的影响 ;形态学方法、流式细胞仪和DNA琼脂糖凝胶电泳观察和分析PC12细胞在转染ClC 3反义寡核苷酸后 ,TG诱导的细胞形态、DNA含量的变化和DNA断裂的情况。结果 与对照组相比 ,反义ClC 3寡核苷酸呈浓度和时间依赖性地抑制ClC 3蛋白的表达 ;使TG诱导的PC12细胞存活率降低 ,凋亡程度加强 ,差异有显著性 ,而正义及随义ClC 3寡核苷酸对此没有影响。结论 反义ClC 3寡核苷酸对TG诱导的PC12细胞凋亡有促进作用。
Objective To investigate the effect of antisense ClC 3 oligonucleotide on thapsigargin-induced apoptosis. Methods The expression of ClC 3 protein was detected by Western blotting. The effect of antisense ClC 3 oligonucleotide on TG-induced cell growth was detected by MTT assay. Morphological methods, flow cytometry and DNA agarose gel electrophoresis were used to observe and analyze PC12 cells transfected with ClC 3 antisense oligonucleotides, TG-induced cell morphology, changes in DNA content and DNA fragmentation. Results Compared with the control group, the antisense ClC 3 oligonucleotide inhibited the expression of ClC 3 protein in a concentration-dependent and time-dependent manner. The survival rate of PC12 cells induced by TG was decreased and the degree of apoptosis was enhanced. The difference was significant Justice and arbitrariness ClC 3 oligonucleotides have no effect on this. Conclusion Antisense ClC 3 oligonucleotide can promote the apoptosis of PC12 cells induced by TG.