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利用正交实验对搅拌式光生物反应器中钝顶螺旋藻(SpirulinaplatensisGeitl)的培养条件即搅拌速度、通气量和光照强度进行优化。实验结果表明:当培养温度为30℃时,通过正交实验所获得的最佳培养条件为搅拌转速120r·min-1,通气量80L·h-1,光照强度5000lx。在最佳培养条件下,收获时螺旋藻的干重为1.922g·L-1。根据回归模型得到相应的优化条件为:光照强度5000lx,通气量150L·h-1,搅拌转速111.70r·min-1,收获量(干重)的预测值为2.293g·L-1。另外,10%的接种量有利于螺旋藻的生长。
The orthogonal experiment was used to optimize the culture conditions of SpirulinaplatensisGeitl in agitated photobioreactor, ie, stirring speed, aeration rate and light intensity. The experimental results showed that when the culture temperature was 30 ℃, the optimum culture conditions obtained by orthogonal experiment were stirring speed 120r · min-1, aeration 80L · h-1, light intensity 5000lx. Under the optimal culture conditions, the dry weight of spirulina at harvest was 1.922 g · L -1. According to the regression model, the corresponding optimal conditions were as follows: light intensity 5000 lx, aeration 150 L · h-1, stirring speed 111.70 r · min-1, and the predicted value of harvest (dry weight) was 2.293 g · L -1. In addition, 10% of the inoculum is conducive to the growth of Spirulina.