This study evaluated the lard enriched with n-3 polyunsaturated fatty acids (PUFAs) on serum lipid composition in rats. The experiment contained two parts: (A) 20 crossed pigs (Landrace×Min, 60±2 kg) were assigned randomly into two dietary groups: a control group and a group fed the diet added 10％ linseed based on the control diet. This part lasted 56 days and the results showed that the levels of the total n-3 PUFA in lard were improved approximately 2-fold (p<0.05), when pigs were fed 10％ flaxseed compared to a control diet of c. The lard taken from pigs was melted and preserved for rat experiment; (B) 45 Sprague-Dawley rats at day 36 were randomly fed one of three diets for 42 days: the diet without added fat, the diet contained 10％ common lard from pigs fed a conventional c ration and the diet contained 10％ lard enriched with n-3 PUFA from pigs fed 10％ flaxseed. Rats fed lard significantly increased (p<0.05) serum cholesterol levels compared with rats maintained on a diet without lard. However, rats fed the lard enriched with n-3 PUFA significantly decreased the level of low-density lipoprotein cholesterol in serum (p<0.05) and better hepatic antioxidant defense capacities, when compared with rats fed common lard (p<0.05). The results showed that pigs fed diet with n-3 PUFA produced lard enriched with n-3 PUFA and thus provided positive health benefits to the consumers.