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目的建立红腺忍冬藤的HPLC指纹图谱,为其质量评价提供理论依据。方法采用RP-HPLC法。色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm),乙腈-0.3%甲酸溶液梯度洗脱,体积流量:1.0 mL.min-1,柱温:30℃,检测波长:238 nm。用国家药典委员会“相似度评价软件(2004A)”处理分析,建立红腺忍冬藤的HPLC指纹图谱。结果确立了16个共有峰的共有模式,10批红腺忍冬藤样品指纹图谱相似度范围在0.927~0.973之间。结论本研究所建立的方法可靠、简便易行。为红腺忍冬藤药材的质量控制提供了科学依据。
Objective To establish the HPLC fingerprints of Honeysuckle hybrids and provide a theoretical basis for their quality evaluation. Methods RP-HPLC method. The column was eluted with a gradient of acetonitrile-0.3% formic acid on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm). The volume flow rate was 1.0 mL.min-1. The column temperature was 30 ℃ and the detection wavelength was 238 nm. With the State Pharmacopoeia Commission “similarity evaluation software (2004A) ” analysis of the treatment, the establishment of HPLC fingerprints of H. eryngii honeysuckle. Results The common pattern of 16 common peaks was established. The similarity of fingerprints of 10 samples of Rhodiola sachalinensis was between 0.927 and 0.973. Conclusion The method established in this study is reliable, simple and easy to implement. It provides a scientific basis for the quality control of Honeysuckle honeysuckle herbs.