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目的研究白介素18(IL-18)基因型及血清水平与2型糖尿病肾病(DN)的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测360例2型糖尿病患者(160例伴DN)及180名正常对照组的IL-18的基因多态性,同时采用酶联免疫吸附试验(ELISA)检测血清IL-18水平。结果与对照组比较,糖尿病肾病组血清IL-18水平显著高于对照组(P<0.01)。IL-18基因-137 G/C多态性在两组人群中的分布差异有统计学意义(P<0.05),携带 137 C等位基因的糖尿病肾病患者血清IL-18水平显著高于不携带者(P<0.05)。结论IL-18基因-137 G/C多态性与DN的发病可能具有相关性,其中C等位基因可能是DN发病的遗传易感基因;携带C等位基因的个体可能通过促进IL-18的高度表达进而增加了DN的发病风险。
Objective To investigate the relationship between interleukin 18 (IL-18) genotypes and serum levels and type 2 diabetic nephropathy (DN). Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect IL-18 gene polymorphism in 360 patients with type 2 diabetes mellitus (160 with DN) and 180 normal controls. Serum IL-18 level was measured by enzyme-linked immunosorbent assay (ELISA). Results Compared with the control group, the level of serum IL-18 in diabetic nephropathy group was significantly higher than that in the control group (P <0.01). The distribution of IL-18 -137 G / C polymorphism in the two groups of patients was significantly different (P <0.05). The serum IL-18 level of patients with diabetic nephropathy carrying the 137 C allele was significantly higher than that without carrying (P <0.05). Conclusions The IL-18 gene -137 G / C polymorphism may be correlated with the pathogenesis of DN. The C allele may be the genetic predisposition to DN. The individuals with C allele may be affected by the promotion of IL-18 The high expression of DN increased the risk of the disease.