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建立了HPLC法同时测定油橄榄叶抗糖尿病有效部位中的5种多酚类化合物含量。优选的色谱条件为:色谱柱:Sinochrom ODS-BP柱(250×4.6 mm,5μm);DAD检测器;检测波长:230 nm;流速:1.0 mL/min;柱温:30℃;进样量:10μL;流动相:水(A)+甲醇(B),梯度洗脱:0~5 min,30%B;5~20 min,30%B~52%B;20~25 min,52%B~50%B。在优选的色谱条件下,5种多酚类化合物能够得到较好的分离。日内RSD为1.4%~2.6%,日间RSD为0.8%~2.8%,回收率为97.1%~113.2%。方法可用于测定油橄榄叶及其抗糖尿病有效部位中的5种多酚类化合物的含量。
A HPLC method was established for the simultaneous determination of five polyphenolic compounds in the anti-diabetic active site of Olive leaf. The chromatographic conditions were: Column: Sinochrom ODS-BP column (250 × 4.6 mm, 5μm); DAD detector; detection wavelength: 230 nm; flow rate: 1.0 mL / min; 10 μL; mobile phase: water (A) + methanol (B), gradient elution: 0 to 5 min, 30% B; 5 to 20 min, 30% B to 52% B; 50% B. Under the optimal chromatographic conditions, five kinds of polyphenols can get better separation. The daily RSD was 1.4% -2.6%, daytime RSD was 0.8% ~ 2.8% and the recovery rate was 97.1% ~ 113.2%. The method can be used to determine the content of 5 kinds of polyphenols in the olive leaf and its anti-diabetic effective parts.